|
KMID : 0613820120220010041
|
|
Journal of Life Science
2012 Volume.22 No. 1 p.41 ~ p.48
|
|
|
Bee Venom Inhibits Angiogenesis by Decreasing HIF-1α Expression in HCT116 Cells
|
|
Shin Jae-Moon
Jeong Yun-Jeong
Park Kwan-Kyu
Choe Jung-Yoon
Lee Kwang-Gill
Han Sang-Mi
Yeo Joo-Hong
Chung Il-Kyung
Chang Young-Chae
|
|
|
Abstract
|
|
|
|
Bee venom (BV) has been used in medicine to treat a variety of diseases including arthritis, rheumatism, and various cancers. Recent reports indicate that BV has anti-angiogenic effects, but the precise molecular mechanism underlying the effects of BV against colorectal cancer remains to be elucidated. We examined the effects of BV and its major components (melittin and apamin) on tumor angiogenesis and found that BV significantly decreased protein levels of hypoxia-inducible factor-1α (HIF-1α), an important factor involved in angiogenesis and tumor progression, in human colorectal carcinoma HCT116 cells. BV also suppressed the transcription of HIF-1α under hypoxia, leading to a decrease in the expression of vascular endothelial growth factor (VEGF), a major target gene of HIF-1α. We also found that these effects were mainly elicited by apamin, but not melittin. BV specifically inhibited the phosphorylation of ERK1/2 without changing the total levels of this protein, but had no effect on kinases of p38/JNK and AKT. Our results suggest that BV may inhibit human colorectal cancer progression and angiogenesis by inhibiting HIF-1α and VEGF expression, thereby providing a novel potential mechanism for the anticancer action of BV.
|
|
|
KEYWORD
|
|
|
Angiogenesis, HIF-1α, VEGF, bee venom
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|